Fig. 4
MiR-34a inhibition mitigates MSC natural senescence in OMSCs. a Representative images showing morphological appearance (scale bar = 50 μm) and EGFP expression. RT-qPCR expression analysis for transduction efficacy of miR-34a reduction in OMSCs. Quantification for cell surface area and cell aspect ratio in anti-NC and anti-34a expressed OMSCs. b Logarithmic proliferation in miR-34a-insufficient OMSCs. c Population doubling time (PDT). d Detection and analysis of cell cycle. e Osteogenic differentiation of MSCs (scale bar = 100 μm) and quantification. f SA-β-gal staining (scale bar = 50 μm) and quantification. g Determination of mRNA expression levels of the senescence markers p16INK4a and p21WAF1/CIP in natural senescent OMSCs after miR-34a inhibition by RT-qPCR; n = 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs. O anti-NC