Fig. 7
MiR-34a level alterations exert on MSC senescence via Nampt-NAD+-Sirt1 axis. a, b SA-β-gal staining (scale bar = 100 μm) (a) and quantification (b) in P3MSCs treated with miR-NC, miR-34a, miR-34a plus LV-Vector or miR-34a plus LV-Nampt. c, d Detection of mRNA expression levels of Nampt (c) and senescence-related factors p16INK4a (d) by RT-qPCR after interfering with miR-NC, miR-34a, miR-34a plus LV-Vector or miR-34a plus LV-Nampt. e–g Determination of NAD+ content (e) and NAD+/NADH ratio (f), as well as Sirt1 deacetylase activity (g) after interfering with miR-34a expression; n = 3 independent experiments. **P < 0.01 vs. miR-NC, miR-34a, P10 anti-NC, or O anti-NC; △P < 0.01 vs. miR-34a, ▲P < 0.01 vs. miR-34a plus LV-Vector