Fig. 3

Relative mRNA expressions of erythroid specific transcription factors in each erythroid differentiation stage. Mouse bone marrow cells were isolated and categorized as R1, R2, R3/Aqp0⁻, R3/Aqp0⁺, R4, and nonerythroid populations on the basis of the fluorescence intensity of three erythroid specific cell surface markers (CD71, TER119, and Aqp0). The mRNA expression level of each gene in the R1 region was normalized to one-fold. Relative folds of mRNA expression of Klf1 (a), Nfe2 (b), and Gfi1b (c) in each cell population relative to R1 were analyzed using a quantitative reverse polymerase chain reaction assay. Data are representative of three independent experiments and reported as the mean ± standard deviation. *P < 0.05 and **P < 0.01 relative to R1 groups