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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: DPSCs treated by TGF-β1 regulate angiogenic sprouting of three-dimensionally co-cultured HUVECs and DPSCs through VEGF-Ang-Tie2 signaling

Fig. 3

T-DPSCs exhibited similar functional properties as SMCs. a Contraction assay of the contractility of T-DPSCs in collagen gel. 1, 2, and 3 are three replicates. b The gel diameter was measured and analyzed using ImageJ software after T-DPSC seeding in the gels for 48 h. c Confocal laser microscopy images showed the location of HUVECs and T-DPSCs in sprouting structures. HUVECs and T-DPSCs were labeled with green and red cell tracker dyes, respectively. Scale bar represents 100 μm. d, e T-DPSCs significantly inhibited the sprouting of HUVECs in a 3D spheroid model. Data are mean ± standard error for n = 3 replicates, **p <0.01, ****p <0.0001

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