Fig. 4

Adhesive molecule expression, migration, maintenance and proliferation of SCAP-ECs. a, b The gene expression levels of IL-6 and IL-8 in SCAP-ECs and HUVECs after LPS treatment. All of the results represent the mean ± SEM of 3 independent experiments (n = 3). d, e The protein expression levels of VCAM-1 and ICAM-1 in SCAP-ECs and HUVECs after LPS treatment. GAPDH was used as an internal control. e, f Transwell migration assay did not demonstrate statistical significance of SCAP-ECs and HUVECs in the number of cells migrated through the membrane. Scale bar = 200 μm. Values are presented as mean ± SD. g Representative bright field photos of maintenance and passaging of SCAP-ECs. Scale bar = 500 μm. h The passage 1 to 3 cells could still express the key endothelial cell markers CD31 and VEGFR2, as demonstrated by western blot. i CCK-8 assay did not show statistical significance in OD450 values in SCAP-ECs after digestion and HUVECs. *P < .05, **P < .01