Fig. 6
From: A novel strategy for isolation of mice bone marrow endothelial cells (BMECs)
Induction of primary bone marrow-derived endothelial cells by tumor necrosis factor-alpha and quantify by RT-qPCR and immunoblotting. a, b The relative mRNA expression molecule of intracellular adhesion molecule -1 (ICAM-1) and vascular adhesion molecule -1 (VCAM-1). c, d The mRNA expression of endothelial junction adherent molecules (ESAM and VE-cadherin) using beta-actin and GAPDH as reference genes. e Western blot imaging of proteins with protein ladder (M). f–h The relative densitometry of VE-cadherin, VCAM-1, and ICAM-1 using GAPDH as endogenous gene ImageJ software. The bar chart indicates the mean± SD by the Student’s t test, (N = 3 experimental repeats for 3 mice per group (RT-qPCR and N=2 immunoblotting six mice per group). *p< 0, **p<0.01, ***p<0.001, ****p<0.0001, and NS not significant