Fig. 2

UMSC are capable of uptake and release GALNS enzyme. a Specific activity of UMSC and deficient fibroblast lysate when cultured in rhGALNS-supplemented media. Both cells showed a strong increase in GALNS activity from 5h to 24h. After the addition of mannose-6-phosphate, both cells showed a decrease in activity, with UMSC showing an 80–90% reduction, and the fibroblasts having a 65–85% reduction after 24 h. b Specific activity of fibroblasts co-cultured with UMSC, normalized to fibroblasts cultured without UMSC. After an initial delay, the fibroblasts displayed a 25% increase in GALNS activity on days 3 and 5, then returning to baseline on day 7. Error bars: mean ± SD. (*P < 0.05, ***P < 0.001, two-tailed unpaired t test with Welch’s correction)