Fig. 4

MMVs improved mitochondrial function of IEC-6. a Representative microphotographs of mitochondria labeled with mitotracker-Red and the average mitochondria length in 30 cells. The scale bar represents 10μm. b The relative expression of Tomm20 the in IEC-6 treated by MMVs. β-actin was used as the internal reference (n=5). c Representative microphotographs of ΔΨm in IEC-6 treated by MMVs. The scale bar represents 10μm. d Representative microphotographs of ROS in IEC-6 treated by MMVs. The scale bar represents 50μm. e ATP concentration in IEC-6 treated by MMVs (n=6). f OCR in IEC-6 treated by MMVs (n=6). g The influence of uncoupling agent DNP on the effect of MMVs on TEER of IEC-6 (n=5). h The influence of DNP on the effect of MMVs on BSA leakage (n=6). i The influence of DNP on the effect of MMVs on CCK8 proliferation assay (n=6). j The influence of DNP on the effect of MMVs on scratch migration assay. The scale bar represents 100μm. k The influence of DNP on the effect of MMVs on the relative expression of PCNA. β-actin was used as the internal reference (n=5). l The concentration of aspartate in IEC-6 treated by MMVs (n=6). m The relative expression of GOT1 in IEC-6 treated by MMVs. β-actin was used as the internal reference (n=5). Ctl, normal control group; LPS, LPS-stimulated group; CMV, CMV-treated group; MMV, MMV-treated group; DNP: MMV+DNP group. **P < 0.01