Fig. 5

In vitro hematopoietic differentiation of a healthy donor and GS-2 iPSCs. Healthy donor and GS-2 iPSCs were co-cultured with hematopoietic differentiation medium (HDM) 1 (left) and 2 (right) for 9 days on the confluent feeder layers of Op9 cells. For specifics of the media, see the main text. a Within 4–5 days, hematopoietic island appeared in separate areas surrounded by stromal Op9 cells. *Unchanged Op9 cells; ^#differentiated Op9 cells; ^&islands of differentiating iPSC/HSCs. b FACS dot plots of the above co-cultures. c Microscopic photograph of colony assays (left) showing prominent white colonies that were confirmed to be CFU-GM by FACS analysis (right)