Fig. 5

MiR-146a/Numb reverses β-cell dedifferentiation and improves β-cell function through β-catenin nuclear translocation. a Nuclear translocation of β-catenin detected after bmMDE treatment in primary islets, as indicated by Western blotting analysis. b, c Western blotting for nuclear translocation of β-catenin in primary islets transfected with miR-146a mimics or treated with an miR-146a inhibitor combined with bmMDEs. Glucose-stimulated insulin secretion (d) in islets transfected with miR-146a mimics combined use XAV-939. e–h RT-qPCR analyses for Pdx1, Foxo1, Ngn3, and Oct4 in islets transfected with miR-146a mimics combined use XAV-939. Confirmation of the changes of β-catenin nuclear translocation of Numb overexpression (i) and siRNA (j). β-actin and GAPDH were used as loading controls. Data are presented as the mean ± SEM (^∗P < 0.05; ^∗∗P < 0.01; ^***P < 0.001)