Table 2 Characteristics of the 21 studies included in meta-analysis

Abraham et al. [51]

C57BL/6, Female, 7–8 weeks

Long-term SD G-CSF; AMD3100; T-140; long-term SD G-CSF + T-140; long-term SD G-CSF + AMD3100

G-CSF, 2.5 μg/mouse s.c. twice daily for 4 days; AMD3100 5 mg/kg s.c. 2 h before harvest; T-140 5 mg/kg s.c. 2 h before harvest

Kubonishi et al. [56]

C57BL/6, male and female, 7–12 weeks

SCA; long-term SD G-CSF; long-term SD G-CSF + SCA

G-CSF, 125 μg/kg s.c. twice daily for 4 days; SCA, 100 mg/kg i.v. 30 min before harvest

di Giacomo et al. [72]

C57BL/6

EP80031; long-term SD G-CSF + EP80031; AMD3100 + EP80031; long-term SD G-CSF + AMD3100; long-term SD G-CSF + AMD3100 + EP80031

EP80031, 15 mg/kg i.v. 1 h before harvest; G-CSF, 2.5 μg/mouse s.c. twice daily for 4 days; AMD1300, 5 mg/kg s.c. 1 h before harvest

Lucas et al. [74]

C57BL/6, male, 8 weeks

Long-term SD G-CSF; long-term SD G-CSF + desipramine; AMD3100; AMD3100 + desipramine; long-term SD G-CSF + reboxetine

G-CSF, 125 μg/kg s.c. twice daily for 4 days; AMD 3100, 5 mg/kg s.c. 1 h before collection; desipramine, 10 mg/kg/day i.p. for 8 days; reboxetine, 5 mg/kg/day i.p. for 8 days

Hoggatt et al. [77]

C57BL/6

Meloxicam; indomethacin; long-term LD G-CSF; AMD3100; long-term LD G-CSF + indomethacin; long-term LD G-CSF + meloxicam; AMD3100 + meloxicam

G-CSF, 50 μg/kg s.c. twice daily for 4 days; AMD 3100, 5 mg/kg i.p. 1 h before collection; meloxicam, 0.5–12 mg/kg s.c. for 4 days; indomethacin, 0.5–2.5 mg/kg s.c. twice daily for 4 days; AH23848, 10 μg per mouse i.p. for 4 days; L-161,982, 10 μg per mouse i.p. for 4 days

Karpova et al. [78]

C57BL/6

POL5551; HD POL5551; AMD3100

POL5551, 5 or 100 (HD) mg/kg i.p. 2 or 4 h before harvest; AMD3100, 5 mg/kg i.p. 1 h before harvest.

Chen et al. [81]

C57BL/6

Long-term SD G-CSF; ML141; long-term SD G-CSF + ML141

G-CSF, 200 μg/kg/day s.c. for 5 days; ML141, 10 μg/kg/day i.p. for 5 days

Ghobadi et al. [82]

C57BL/6

Bortezomib; long-term SD G-CSF; AMD3100; long-term SD G-CSF + Bortezomib; AMD3100 + Bortezomib

Bortezomib, a single dose of 0.8mg/kg i.v.; G-CSF, 250 μg/kg/day s.c. for 4 days; AMD3100, 5 mg/kg s.c.

He et al. [83]

C57BL/6, 8–10 weeks

10d-FLT-3L; short-term SD G-CSF + AMD3100; 10d-FLT-3L + AMD3100

FLT-3L, 350 μg/kg/day i.p. for 10 days; G-CSF, 150 μg/kg/day i.p. for 5 days; AMD3100, 5 mg/kg i.p. 1 h before harvest

Saez et al. [84]

C57BL/6, male, 6–12 weeks

Long-term SD G-CSF; long-term SD G-CSF + heparin; G-CSF + Anti-VCAM-1 Ab; AMD310; heparin; AMD3100 + heparin

G-CSF, 125 μg/kg s.c. twice for 4 days; heparin, 100 U i.p. 1 h before harvest; Anti-VCAM-1 Ab, 2 mg/kg/day i.v. for 3 days; AMD3100, 5 mg/kg s.c. 1 h before harvest

Zhang et al. [85]

C57BL/6

Me6; AMD3100; long-term SD G-CSF; long-term SD G-CSF + AMD3100; long-term SD G-CSF + Me6

Me6, 5 mg/kg s.c. 12 h before harvest; AMD3100, 5mg/kg s.c. 1 h before harvest; G-CSF, 2.5 μg per mouse s.c. twice daily for 4 days

Forristal et al. [86]

C57BL/6, male, 9–12 weeks

Long-term SD G-CSF; long-term SD G-CSF + AMD3100; long-term SD G-CSF + FG-4497; long-term SD G-CSF + AMD3100 + FG-4497

G-CSF, 125 μg/kg s.c. twice daily for 4 days; AMD3100, 5 mg/kg s.c. 1 h before harvest; FG-4497, 20 mg/kg/day i.p. for 3 days

Lu et al. [91]

C57BL/6, male, 6–8 weeks

LECT2; AMD3100; AMD3100 + LECT2

LECT2, 300 μg/kg/day s.c. for 5 days; AMD 3100, 5 mg/kg s.c. 1 h before collection

Karpova et al. [94]

C57BL/6

HD POL5551; 14d-HD POL5551; 14d-HD AMD3100; 14d-ALT1188; 14d-HD POL5551 + HD AMD3100; 14d-HD POL5551 + CWHM-823

POL5551, 100mg/kg i.p. as a single dose or as continuous infusion for 2 weeks via subcutaneously implanted pumps; ALT1188, 33 mg/kg i.p. as a single injection or as continuous infusion for 2 weeks; AMD3100, 20 mg/kg i.p. as a single injection or as continuous infusion for 2 weeks; CWHM-823, 3 mg/kg i.p.

Ogle et al. [96]

C57BL/6, male, 8–12 weeks

VPC01091; AMD3100; AMD3100 + VPC01091

AMD3100 5mg/kg i.p. 1.5 h before harvest; VPC01091 5mg/kg i.p. 1.5 h before harvest

Adamiak et al. [104]

C57BL/6, 4–6 weeks

Short-term LD G-CSF; AMD3100; short-term LD G-CSF + ARL67156; AMD3100 + ARL67156; short-term LD G-CSF + AMPCP; AMD3100 + AMPCP

G-CSF, 100 μg/kg/day s.c. for 3 days; AMD 3100, 5 mg/kg i.p. 1 h before collection; ARL67156, 2 mg/kg i.p.; AMPCP, 4 mg/kg i.p.

Alt et al. [105]

C57BL/6, Male, 6–10 weeks

Short-term SD G-CSF; AMD3100; short-term SD G-CSF + AMD3100; IL-33; short-term SD G-CSF + IL-33; AMD 3100 + IL-33; short-term SD G-CSF + AMD3100 + IL-33

G-CSF, 200 μg/kg/day s.c. for 3 days; AMD 3100, 5 mg/kg i.p. 1 h before collection; IL-33, 0.04 mg/kg/day i.p. for 3 days

Bisht et al. [106]

C57BL/6, male, 8–9 weeks

Short-term SD G-CSF; short-term SD G-CSF + FG-4497

G-CSF, 125 μg/kg s.c. twice daily for 2 days; FG-4497, 20 mg/kg/day i.p. for 3 days;

Liu et al. [108]

C57BL/6

CASIN; AMD3100; CASIN + AMD3100

CASIN, 1.2mg/kg i.v. 2 h before harvest; AMD3100, 5mg/kg i.p. 2 h before harvest.

Müller et al. [109]

C57BL/6

AMD3100; AMD3100 + LGB321

AMD3100, 5 mg/kg s.c.; LGB321, 100 mg/kg s.c.

Fang et al. [112]

C57BL/6

HF51116; AMD 3100; long-term SD G-CSF; G-CSF + HF51116; long-term SD G-CSF + AMD 3100

G-CSF, 100 μg/kg every 12h s.c. for 4 days; AMD 3100, 5 mg/kg s.c.; HF51116, 5 mg/kg s.c.