Fig. 6From: The Rotary Cell Culture System increases NTRK3 expression and promotes neuronal differentiation and migratory ability of neural stem cells cultured on collagen spongeThe transplantation of NSCs in rats with spinal cord injury. a Spinal cord injuries (with lengths of 3 mm) were inflicted in rats (left). Functional collagen sponge scaffolds with or without cells were precisely placed into the injury site (middle). Samples of the spinal cord tissue following perfused 4% paraformaldehyde fixation (right). b The BBB scores of various groups. Locomotor function recovery in five groups at 1, 2 and 3 weeks after spinal cord injury. c Images of immunofluorescence staining of spinal injury sections at 2 weeks from different groups of SCI rats, stained with antibodies against GFAP (red) and with DAPI (blue). The green signal represents the GFP-labelled transplanted NSCs. Scale bars: 500 μm. d Enlarged images of the migrated GFP+ cells in the RCCS and RCCS+NT-3 groups. Quantification of the number of migrated cells showing that the NSCs in RCCS+NT-3 group possess the best migratory ability in SCI animals. Scale bars: 250 μm. Data represent mean±S.D. from three independent experiments. ***p < 0.001Back to article page