Fig. 6
hAMSC transplantation enhanced oxidation resistance and promoted Ampk/Foxo3a signaling in ovary of AR-DOR mice. a-d Expression levels of proteins in mouse ovary were determined by Western blot analysis. a The expression of antioxidative Sod2 was increased in the hAMSC group. b The expression level of p-FoxO3a and FoxO3a in the hAMSC group was similar to that of NS and HSA groups, but ratio of p-FoxO3a/FoxO3a was significantly improved in the hAMSC group. c The expression of Ampk was significantly promoted in the hAMSC group. d No obvious difference of p-Akt and Akt expression was observed between groups. e Expression of FoxO3a in oocyte were determined by immunofluorescence experiments. stronger signals were observed in the hAMSC group. NS: normal saline group; HSA: 1% human serum albumin group; hAMSCs: hAMSC transplantation group (7.5×106 cells/kg body weight). Scale bar 100 μm. n = 6. Error bars indicate SEM. Different lowercase letters represent significantly statistical differences (P < 0.05)