Fig. 6

MiR-380-5p regulates the abnormal differentiation of IECs via Rspo3 in the DM state. a The dual-luciferase reporter assay demonstrated that the miR-380-5p mimic and the Rspo3-3′-UTR-wt plasmid caused a significant decrease in the luciferase activity. b, c AgomiR-380-5p administration in the db/db mice reduced the overexpressed levels of Rspo3 and Lgr5 after agomiR-380-5p administration in the db/db mice, as shown by Western blot. d Immunohistochemistry showing Rspo3 expression in the db/db mice after agomiR-380-5p administration. e RT-qPCR showed substantially increased mRNA expression of SI, Tff3, and Lyz1 and decreased mRNA expression of ChgA in the db/db mice, which was rescued by the administration of agomiR-380-5p. f, g SI, Tff3, and Lyz1 protein overexpression was substantially decreased, and the levels were similar to the levels observed in the db/+ mice after agomiR-380-5p administration. Additionally, the downregulated ChgA protein expression was partially normalized after agomiR-380-5p administration to levels that were comparable to those observed in the db/+ mice. Scale bar, 50 μm; mean ± SD, n = 6; *P < 0.05 vs db/+-NS; ^#P < 0.05 vs db/db-NS