Table 1 Summary of the technique details of 14 operated eyes of NHPs. Of the 13 animals, 12 had unilateral surgery in the right eye. Case numbers #8 and #14 refer to an animal that had bilateral surgery, with respective procedures performed 2 weeks apart. Thus, in total, 11 animals received a subfoveal hESC-RPE graft, and 3 NHPs had sham surgery/controls, without implantation of hESC-RPE
From: Submacular integration of hESC-RPE monolayer xenografts in a surgical non-human primate model
Case ID | Condition to create bleb (injection method/tamponade) | hESC-RPE graft under fovea | Ophthalmic follow-up | Histology/nuclei count | IHC | Surgical outcome group |
|---|---|---|---|---|---|---|
#1 | Manual injection/BSS | No/sham control | Yes | No, sample processing unsuccessful | No | N. A |
#2 | Manual injection/BSS | No/sham control | Yes | No, sample processing unsuccessful | No | N. A |
#3 | Automated injection/BSS | Yes | Yes | No, sample processing unsuccessful | No | Unfavorable |
#4 | Automated injection/BSS | Yes | No, died at 2 weeks | No, sample processing unsuccessful | No | N. A |
#5 | Automated injection/BSS | Yes | Yes | No, sample processing unsuccessful | No | Unfavorable |
#6 | Automated injection/BSS | Yes | Yes | Yes | Yes | Unfavorable |
#7 | Manual injection/BSS | Yes | Yes | No, foveal sections not obtained | Yes | Favorable |
#8 | Manual injection/air | Yes | Yes | Yes | Yes | Favorable |
#9 | Manual injection/air | Yes | Yes | Yes | Yes | Favorable |
#10 | Manual injection/PFCL | Yes | Yes | Yes | Yes | Favorable |
#11 | Manual injection/PFCL | Yes | Yes | Yes | Yes | Favorable |
#12 | Manual injection/PFCL (Ca2+, Mg2+-free BSS) | Yes | Yes | Yes | Yes | Unfavorable |
#13 | Manual injection/PFCL | Yes | Yes | Yes | Yes | Unfavorable (transition case) |
#14 | Manual injection/air | No/sham control | Yes, euthanized at 6 weeks | No, sample processing unsuccessful | No | N. A. |