Fig. 5
From: Methylsulfonylmethane enhances MSC chondrogenic commitment and promotes pre-osteoblasts formation
MSM modulates gene expression in zebrafish larvae. Increased expression of sp7 gene and reduced tnfrsf1b gene expression in larvae at 9 dpf (A). The fluorescence density produced by calcein staining was higher in larvae after 7 days of MSM treatment compared to untreated larvae (B) (a: control; b: MSM treated). Increased runx2b, sp7, col1a1, and reduced tnfrsf1b expression observed after 14 days of MSM treatment compared to control (C). Bone mineralization evaluated by calcein staining was higher in MSM-treated larvae compared to untreated larvae after 14 days of treatment as well (5D) (a: control; b: MSM treated). *p<0.05; **p<0.01