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Table 2 Grouping according to different electroporation conditions

From: SiRNA in MSC-derived exosomes silences CTGF gene for locomotor recovery in spinal cord injury rats

Conditions/groups

1

2

3

4

5

6

7

8

Voltage (v)

400

400

400

400

400

 

400

400

Resistance (Ω)

50

50

50

50

25

 

150

150

Capacitor (μF)

500

200

300

400

500

 

500

500

Exosome volume (μL)

7

7

7

7

7

7

21

21

siRNA volume (μL)

3.75

3.75

3.75

3.75

3.75

3.75

10

20

  1. SiRNA concentration, 20μM; exosome concentration, 2μg/uL
  2. System, 3.75 μL, 20 μM, siRNA+ 7 μg exosome; the rest volume was 150 μL supplemented with buffer
  3. Group 6 is the control group without electroporation