Fig. 4
hPMSCs enhance Nrf2 expression but inhibit the activation of NF-κB in the GVHD mouse liver tissue. On day −5, Balb/c mice were conditioned using X-ray irradiation (8Gy); then, bone marrow cells and splenic mononuclear cells obtained from C57BL/6J mice were transplanted into the irradiated mice via injection into the tail vein. After 5 days, the mice demonstrated characteristics of GVHD at its peak (GVHDhigh). Concurrently, PBS and hPMSCs were used to treat the GVHDhigh mice. The mice were then sacrificed to harvest the spleen and liver after treatment for 7 days for western blotting (WB). WB was performed to determine the expression of Nrf2, HO-1, NQO1, GCLC, and GCLM in the liver (a, b) and spleen (c, d) of GVHD mice in different groups (n = 5). WB was performed to determine the expression of phosphorylated NF-κB and I-κB in the liver (e, f) and spleen (g, h) of GVHD mice in different groups (n = 5). The results were obtained from three independent experiments *P <0.05, **P < 0.01