Skip to main content
Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Plant callus-derived shikimic acid regenerates human skin through converting human dermal fibroblasts into multipotent skin-derived precursor cells

Fig. 2

NeuSKPCs are induced by treatment of shikimic acid. a Scheme of generation of NeuSKPCs. b Morphology of NeuSKPCs. Nestin promoter GFP was activated in the shikimic acid-treated groups (scale bar = 500 μm). c Western blot analysis was conducted using an anti-nestin antibody (upper panel) in shikimic acid untreated or treated spheroids, and the quantified result is shown in the lower panel (*p < 0.05). d qRT-PCR analysis of SKP-associated genes, nestin, fibronectin, vimentin, pluripotency-associated gene, Sox2, fibroblast-specific genes, Col1A1, and PDGFRa. It was normalized to 18s RNA (N = 3 in each group, **p < 0.01, ***p < 0.001 compared with the control)

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512