Fig. 4

Shikimic acid-induced NeuSKPCs can differentiate into ectodermal and mesodermal lineage. a Scheme of differentiation for NeuSKPCs. b Lipid drops of adipogenic differentiated cells were stained by Oil red O (upper panel, scale bar = 100 μm). c Accumulated calcium of osteogenically differentiated cells were stained by von Kossa staining (scale bar = 200 μm). d qRT-PCR analysis of adipocyte-specific genes, CEBP, PPAR, and FABP (N = 3 in each group; ns, not significant, *p < 0.05, **p < 0.01). e qRT-PCR analysis of osteocyte-specific genes, Runx2, osteopontin, and ALP (N = 3 in each group, *p < 0.05, **p < 0.01). f Differentiated neural cells were stained with MAP2 (green), Tuj1(red), and NeuN (magenta) (scale bar = 100 μm). g qRT-PCR analysis of neuron-specific genes, Tuj1, and NeuroD1 (N = 3 in each group, **p < 0.01)