Fig. 2

MMP3 expression and secretion is increased in TMSCs compared to other MSCs. a Nano-liquid chromatography with tandem mass spectrometry analysis was performed using TMSC-CM, BMMSC-CM, and ADMSC-CM. Peptides of ECM-modulating enzymes are listed. Detected peptides are depicted in black. b The mRNA expression levels of MMP3, MMP1, CTSB, and PEPD in human TMSCs, BMMSCs, and ADMSCs were determined using real-time quantitative PCR (RT-qPCR) with custom oligos. GAPDH was used as the reference gene. c MMP3 protein expression levels in MSC lysates were determined by quantitative western blot analysis by dividing the pixel densities of the MMP3 bands by the pixel densities of the corresponding β-actin bands. d MMP3 protein concentrations in MSC-CM were quantified using a human MMP3 ELISA kit. The data represent the mean ± S.E.M. and were analyzed using one-way ANOVA (*P < 0.05)