Fig. 1

Induction of HUMSCs into HLCs. A Schematic diagram of the reprogramming process. B Sequential changes in cell morphology during the induction process. (I) some apoptotic cells appeared on the 6th day. (II) Short-spindle cells showed on the 9th day. (III) Cells with irregular shape showed on the 12th day. (IV) Epithelial cell clones appeared as shown in the dotted line on the 18th day. C, D Interference efficiency of shRNAs. C c-Myc expression in HUMSCs with/without shRNA interference detected by qPCR. D The knockout efficiency of sh1, sh2, and sh3 on c-Myc detected by Western blot. E Images shown epithelial cell clones in two induced groups of HUMSCs: 3TFs and 3TFs+c-Myc. Scale: 100μm. F Number of epithelial cell clones in three induction groups: 3TFs, 3TFs+c-Myc, and 3TFs+c-Myc+ sh-c-Myc. *P <0.05, t test. The data presented as mean±SD. U.D. stands for undetected. G Flow cytometry analysis of HUMSC-iHeps infected with TTR-mCherry lentivirus