Fig. 3

HUMSC-iHep possessed hallmark functions of hepatocytes. A Basic liver function analysis of HUMSC-iHeps, including PAS staining and uptake of ICG and LDL. Scale bar: 100 μm. B, C Secretion of ALB (B) and AAT (C) increased during the induction process as measured by ELISA. D The mRNA levels of CYP genes in HUMSCs, HUMSC-iHeps, and PHHs before inducer treatment were determined by qPCR. Data normalized to the levels in PHH. *P < 0.05, t test. Data are represented as the mean ± SD. UD undetectable. E The mRNA levels of the induced CYP enzymes were measured by qPCR. CYP1A1 and CYP1A2 were induced by 3-methylcholanthrene. CYP2A1 and CYP3A1 were induced by phenobarbital. CYP2E1 was induced by acetone. Fold induction in HUMSC-iHeps and PHHs was normalized to expression in corresponding cells without inducer treatment. *P < 0.05, t test. Data are represented as the mean ± SD. F Expression of drug transporter genes in HUMSC-iHeps determined by qPCR. Data are normalized to PHHs. *P < 0.05, t test. Data are represented as the mean ± SD