Fig. 3
From: SOX9 inactivation affects the proliferation and differentiation of human lung organoids
SOX9−/− does not affect the maturation of airway organoids. A Schematic of the protocol for differentiation of organoids from day 21 NKX2.1+ lung progenitors. B Bright-field images of SOX9 wild-type (WT) and knockout (SOX9−/−) airway organoids on days 35 and 48. Scale bars, 500 μm. C mRNA expression levels of differentiation markers (n = 3, representative of 3 separate experiments). NKX2.1, LP marker; P63, basal cell marker; FOXJ1, ciliated cell marker; SCGB1A1, club cell marker; MUC5AC, goblet cell marker. D Immunofluorescence labeling of airway markers in organoids on day 35. Scale bars, 500 μm (left); 500 μm (right, WT); 100 μm (right, SOX9−/−)