Fig. 5

Comparative immunostaining of the cerebellum between WT and Jdp2-KO mice. Immunohistochemical analysis of cerebellum in WT and Jdp2-KO mice at P6 was performed using anti-Atoh1, and Slc7a11 proteins. a Cerebellum from Jdp2-KO and WT mice were stained for Atoh1. The ratio of Atoh1-stained cells to the total number of cells was estimated using the immunohistochemistry-stained-slides as described previously [18]. Extended Figure-panels in Atoh1 (brown color) and Slc7a11 (green color) IHC sample were shown as the marks inside and outside to focus upon the staining in GCPs and Purkinje cells [43]. The section number was 6; the sample number was 3 mice for WT 129-C57/BL6J and 3 mice for Jdp2-KO 129-C57/BL6J mice. * p < 0.05, ** p < 0.01. b Comparative expression of antioxidation-related proteins in WT and Jdp2-KO cerebellum from mice at 9 weeks, P6, and GCPs (n = 5). The western blotting data from GCPs derived from WT and Jdp2-KO mice were the same to those previously reported [18]. Slc7a11 35 kDa protein was an unubiquitinated form and Slac7a11 55 kDa protein is ubiquitinated form as previously reported [18]. The right lane indicates the apparent molecular weight. The relative expression based on β-actin was calculated in the lower panel of each. Western blotting was carried out as described in the “Materials and methods” section. c. Statistical analysis of the data from Western blotting as sown in panel b. n = 5. The statistics analysis was performed as describe in the “Materials and methods” section. ** p < 0.01, *** p < 0.005