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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Nicotinamide promotes pancreatic differentiation through the dual inhibition of CK1 and ROCK kinases in human embryonic stem cells

Fig. 3

Inhibition of CK1α and CK1ε promotes pancreatic progenitor induction. a RT-qPCR analysis of pancreatic progenitors on day 13 treated with D4476, PF4800567 and LH846 to measure mRNA levels of NKX6.1, PDX1, PTF1A and NGN3 (n > 3), *p < 0.05; b Flow cytometry analysis of pancreatic progenitors on day 13 for PDX1+ and NKX6.1+ percentage under Mock, D4476, PF4800567 and LH846 conditions (n = 3); c RT-qPCR analysis of pancreatic progenitors on day 13 induced from shCK1α, shCK1δ and shCK1ε cell lines to measure mRNA level of NKX6.1 (n = 3), *p < 0.05; d Flow cytometry analysis of pancreatic progenitors on day 13 induced from shCK1α, shCK1δ and shCK1ε cell lines for percentage of PDX1+ / NKX6.1+ (n = 3)

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Stem Cell Research & Therapy

ISSN: 1757-6512