Fig. 1

Early passaged human ESC-derived NPC grafts survive in immunocompetent adult rats up to 12 weeks post-transplantation (wpt). A Verification of healthy human NPCs and graft expansion by co-localization of DsRed reporter and human-specific nuclear antigen. Scale bar, 50 μm. B A representative picture of massive human cell death occurred at late time points in some recipients, showing lost DsRed reporter and human-specific nuclear antigen. C Population data showing that all human grafts survive healthily within 4 wpt, and a late-onset rejection occurs thereafter with a rejection rate more than 50% at 12 wpt for early passaged NPCs