Fig. 4

Late passaged human NPCs with low proliferation survive much longer than early passaged NPCs. A, B Ki67 immunostaining showing relative high proliferation of early passaged NPCs (A) and very limited proliferation of late passaged NPCs (B) at 4–8 wpt. Scale bar, 50 μm. C Statistical analysis of proliferation levels of early and late NPCs at 4 wpt (unpaired T-test, P < 0.01**). N = 3 biologically independent samples. D Comparative analysis of graft survival time of early and late passaged NPCs (log-rank test, P < 0.01**)