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Table 1 Composition of cell culture medium

From: Schwann cells promote prevascularization and osteogenesis of tissue-engineered bone via bone marrow mesenchymal stem cell-derived endothelial cells

Culture medium Composition Manufacturer’s name and location
SC regular medium DMEM containing:
10% FBS
2 μM forskolin
10 ng/ml heregulin-β-1
HyClone, Thermo Fisher Scientific, Pittsburgh, PA Gibco, USA
Gibco, Gaithersburg, MD
BioVision, Milpitas, CA
PeproTech, Rocky Hill, NJ
SC medium for purification DMEM containing:
10% FBS
10 μM Ara-C
Hyclone
Gibco
Sigma-Aldrich, St. Louis, MO
AEC medium M199 containing:
5% FBS
20 μg/ml ECGS
100 μg/ml heparin
Hyclone
Gibco
ScienCell, Carlsbad, CA
Sigma-Aldrich
BM-MSC medium DMEM containing:
10% FBS
Hyclone
Gibco
IEC induction medium DMEM (4.5 g/l glucose) containing:
EGM-2 SingleQuots
Hyclone
Lonza, Basel, Switzerland
IOB induction medium DMEM (4.5 g/l glucose) containing:
10 nM dexamethasone
50 μg/ml ascorbic acid
10 mM sodium β-glyceryl phosphate
Hyclone
Sigma-Aldrich
Sigma-Aldrich
Sigma-Aldrich