Table 1 Composition of cell culture medium
Culture medium | Composition | Manufacturer’s name and location |
|---|---|---|
SC regular medium | DMEM containing: 10% FBS 2 μM forskolin 10 ng/ml heregulin-β-1 | HyClone, Thermo Fisher Scientific, Pittsburgh, PA Gibco, USA Gibco, Gaithersburg, MD BioVision, Milpitas, CA PeproTech, Rocky Hill, NJ |
SC medium for purification | DMEM containing: 10% FBS 10 μM Ara-C | Hyclone Gibco Sigma-Aldrich, St. Louis, MO |
AEC medium | M199 containing: 5% FBS 20 μg/ml ECGS 100 μg/ml heparin | Hyclone Gibco ScienCell, Carlsbad, CA Sigma-Aldrich |
BM-MSC medium | DMEM containing: 10% FBS | Hyclone Gibco |
IEC induction medium | DMEM (4.5 g/l glucose) containing: EGM-2 SingleQuots | Hyclone Lonza, Basel, Switzerland |
IOB induction medium | DMEM (4.5 g/l glucose) containing: 10 nM dexamethasone 50 μg/ml ascorbic acid 10 mM sodium β-glyceryl phosphate | Hyclone Sigma-Aldrich Sigma-Aldrich Sigma-Aldrich |