Fig. 3

CD201 expression marks a cluster of functional HSC/HSPC in our 3D self-assembling peptide-mediated OP9 coculture hematopoietic induction system. A FACS analysis of the percentage of LSK and LSKCD201⁺ cells derived from 3D+OP9 and 3D+0.1% gelatin group on day 5. B Statistical analysis of percentage and absolute numbers LSKCD201⁺ cells of the 3D+OP9 and 3D+0.1% gelatin co-culture system on days 2, 5, and 7 respectively (n = 3). C Sorted Lin⁻c-kit⁺CD201⁺ cells were analyzed by a CFU assay. Representative field images of the different types of colonies obtained from Lin⁻c-kit⁺CD201^- and Lin⁻c-kit⁺CD201⁺ cells and cellular morphology of colonies by May-Giemsa staining. Arrowheads depict indicated morphology. E, erythrocyte; M, macrophage; Mk, megakaryocyte. Scale bar =100μm. D Lin⁻c-kit⁺CD201⁺ cells formed a significantly higher number of colonies than Lin⁻c-kit⁺CD201^- cells. E Statistical analysis of the variety of colonies between Lin⁻c-kit⁺CD201^- cells and Lin⁻c-kit⁺CD201⁺ cells. Error bars represent mean ± SD of samples from at least three independent experiments (n = 3). NS, not significant; *p < 0.05, **p < 0.01