Fig. 1

Characteristics of human amniotic mesenchymal stem cells (hAMSCs). a hAMSCs showed a spindle-like and fibroblastic-shaped morphology (scale bar = 100 μm). b The expressions of the featured makers in hAMSCs were detected by flow cytometric analysis for mesenchymal stem cells (CD73, CD90, CD29, CD105), hematopoietic stem cells (CD34, CD45) and human leukocyte antigen class I (HLA-I ABC), co-activator cytokine (CD40, CD80), and human leukocyte antigen class II (HLA-II DR). The expression of each antigen was presented with the corresponding isotype control (tube 1 in red). c The expressions of various markers were confirmed by PCR: embryonic stem cell surface markers of SOX2, Nanog, and OCT4; mesenchymal stem cell markers of CD29, CD90, CD73, and CD105 and hematopoietic stem cell markers of CD34, CD133, and CD45. d SSEA4 (embryonic stem cells surface marker) and Vimentin (mesenchymal stem cells marker) were detected by immunofluorescent staining in hAMSCs (scale bars, 20 μm)