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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Human amniotic mesenchymal stem cells-conditioned medium protects mice from high-fat diet-induced obesity

Fig. 5

Effects of hAMSCs-CM on differentiation of pre-adipocytes 3T3-L1. a the lipid deposition was detected by Oil-O Red staining in pre-adipocytes 3T3-L1 after 14 days differentiation induction (scale bars, 100 μm). b–e the mRNA expressions of AP2, C/EBPα, PPARγ, and SREBP1 were examined by RT-qPCR. f The protein levels of C/EBPα, FASN, and PPARγ in 3T3-L1 cells were determined by western blot (GAPDH served as the loading control). g-i The protein levels of C/EBPα, FASN, and PPARγ were quantitatively analyzed. Each experiment was repeated three times. Data represent as means ± SD. *p< 0.05; **p< 0.01, ***p< 0.001

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Stem Cell Research & Therapy

ISSN: 1757-6512