Fig. 6

Effects of hAMSCs-CM on HFD-induced inflammation in WAT of the obese mice. a–d The mRNA expressions of the pro-inflammatory cytokines CD11C and TNFα (a, b) and the anti-inflammatory cytokines ARG-1 and IL10 (c, d) were detected by RT-PCR in WAT. e F4/80 expressions were determined by IF staining in WAT (scale bars, 20 μm). f The protein levels of P-STAT3, STAT3, and ARG-1 were detected by western blot. g, h The ratio of P-STAT3/STAT3 and the quantitative Arg-1 were analyzed in WAT (β-actin served as the loading control). i–l LPS+IFNγ or IL4-mediated the mRNA expressions of IL6, IL-1β, IL10 and ARG-1 were examined by RT-PCR in RAW264.7 cells treated with or without hAMSCs-CM. Each experiment was repeated three times. Data represent as means ± SD. *p< 0.05; **p< 0.01, ***p< 0.001