Fig. 6

MiR-100-5p inhibited osteogenesis of hBMSCs and angiogenesis of HUVECs, promoting adipogenesis of hBMSCs. A RT-PCR was used to detect the expression of miR-100-5p in hBMSCs. B WB was used to detect the expression of BMPR2, collagen type 1, OCN, RUNX2, ALP, OPN, and PPARγ in hBMSCs. C The expression of miR-100-5p in HUVECs. D WB was used to measure the expression of BMPR2, FGF2, and VEGFA in HUVECs. E ALP staining of hBMSCs (scale bar = 100 μm). F Alizarin Red S staining of hBMSCs after cultured in ODM for 14 days. G Oil Red O staining of hBMSCs (scale bar = 50 μm). H Tube formation assay of HUVECs (scale bar = 200 μm). NM-CTL, controls for differentiation. *P < 0.05, versus NC group; #P < 0.05, versus agomiR-100-5p group. All data were expressed as mean ± SEM