Fig. 2

Flow cytometric analysis of oxidative stress and mitochondrial membrane potential in iPS-MSCs and iPS-MSC^dOex-mIRs and IF microscopic findings. A Fluorescent intensity of DCFDA (i.e., an indicator of total intracellular oxidative stress), * vs. other groups with different symbols (†, ‡), p< 0.0001. B Fluorescent intensity of Mito-SOX (i.e., indicator of mitochondrial oxidative stress), * vs. other groups with different symbols (†, ‡), p< 0.0001. C Fluorescent intensity of TMRE (i.e., an index mitochondrial membrane potential), * vs. other groups with different symbols (†, ‡, §), p< 0.0001. n=8 for each group. D–G Illustrating the immunofluorescent microscopic finding (400×) for identification of γ-H2AX+ cells (pink color). H Analytical result of positively stained γ-H2AX cells, * vs. other groups with different symbols (†, ‡), p< 0.001. n=4 for each group. Scale bars in the lower right corner represent 20 μm. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test. Symbols (*, †, ‡) indicate significance (at 0.05 level). MFI = mean fluorescent intensity; G1= iPS-MSC; G2 = iPS-MSC^dOex-mIRs; G3 = iPS-MSC + H₂O₂/100uM; G4 = iPS-MSC^dOex-mIRs + H₂O₂/100uM