Fig. 2
From: Adipose-derived stem cells therapy effectively attenuates PM2.5-induced lung injury
ADSC transplantation attenuated PM2.5-induced pulmonary oxidative stress and cell apoptosis. A, B After PM2.5 exposure and ADSC transplantation, the levels of 3′-nitrotyrosine (3′-NT) (A) and 4-hydroxynonenal (4-HNE) (B) in lung tissue were measured. C–E Lung sections from the control and PM2.5-exposed mice were stained with dihydroethidium (DHE) or DAPI (blue) and dye (red) from a TUNEL assay kit (scale bar = 50 μm, the arrows point to TUNEL-positive cells). The relative DHE fluorescence intensity (D) and TUNEL-positive cells (E) were quantified. F Lysates of lung tissue were subjected to western blotting to determine the expression levels of Bax, Bcl-2, cleaved caspase-3, superoxide dismutase 1 (SOD1), and peroxiredoxin 4 (PRDX4). β-Tubulin was used as a loading control. In A–E, N = 5; in F, N = 3; the data are presented as the mean ± SD; asterisk indicates p < .05; double asterisks indicate p < 0.01