Fig. 3

Expression of LINGO-1 by sp-NSPCs and differentiated cells. A LINGO-1 mRNA and B LINGO-1 protein expression levels increased significantly during differentiation, as revealed by quantitative real-time PCR and Western blot analyses. Double immunostaining shows C LINGO-1/Nestin-positive sp-NSPCs cultured in NSPC growth media and differentiated into D LINGO1/Tuj-1-positive neurons, LINGO-1/GFAP-positive astrocytes, and LINGO-1/O4-positive oligodendrocytes (arrows) when the medium was changed to the differentiation medium. Enlarged areas show cells at higher magnification. Nuclei were stained with DAPI (blue) (bar = 20 μm), ^*P < 0.05. Results are expressed as the mean ± SD. All of the analysis was calculated based on three biological replicates