Fig. 6

Wnt5a is the target of miR-15b-3p. A miR-15b-3p binding site in the 3′UTR of Wnt5a, as predicted by TargetScan. B, C Validation of miRNA binding to the 3′UTR of Wnt5a by luciferase activity assay. Reduction in luciferase activity demonstrated binding of miR-15-3p to the 3′UTR of Wnt5a. D–F Downregulation of Wnt5a protein levels by overexpression of miR-15b-3p in sp-NSPCs results in less neurons and more astrocytes, based on immunofluorescence staining and quantification. G–I Upregulation of Wnt5a protein levels after LNA inhibition of miR-15b-3p in sp-NSPCs results in more neurons but less astrocytes (bar = 100 μm), ^*P < 0.05. Results are expressed as the mean ± SD. All of the analysis was calculated based on three biological replicates