Fig. 7

Bmi-1 and Trx were important members of PCG/TrxG protein and knocking down Bmi-1/Trx downregulated TOPOIIα/UHRF1 expression. a, b HSPCs from 4-week-old mice were transfected with Bmi-1-siRNA and Trx-siRNA at 48 h. Bmi-1 gene expression (b, c) and Trx gene expression (a, d) were validated by real-time PCR and WB analysis. mRNA expression of TOPOIIα (e) and UHRF1 (f) decreased significantly in the Bmi-1 or the Trx knocked down group. SA-β-gal-stained cells increased in the Bmi-1 or the Trx knocked down group, but there was no significant difference (h). The colony-forming ability of HSPCs significantly decreased in the Bmi-1 (j) or the Trx (i) knocked down group compared with the control group