Fig. 3

Blocking CCR2-CCL2 interaction lead to reverse the polarization of T cells toward CCR2+CD4+ T cells in vitro. The CCL2 neutralizing antibody was added to block CCL2 interaction with CCR2 in the co-culture system. Flow cytometry was used to detect a T cell activation, b T cell proliferation, c CCR2 expression on T cells in contacting culture system and d transwell system, e the proportion of CCR2+CD4+ T cells in contacting culture system and f transwell system. n = 5, *P <0.05; **P <0.01; ***P <0.001