Fig. 6
From: Embryonic stem cell-derived extracellular vesicles promote the recovery of kidney injury
ESC-EV treatment enhances the physiological function of renal tubular epithelial cells. a Representative images show Ki-67 immunostaining (red) detected in renal tubular epithelial cells (LTL, green) at day 3 after AKI. Scale bar represents 100 μm. b Quantitative analysis of Ki-67 immunostaining at day 3 after AKI. Quantitative analysis of 5 random fields in tissue sections of 5 different mice. c Representative immunofluorescence images of γ-H2AX expression in HK-2 cells with and without H2O2 pretreatment. Scale bar represents 100 μm. d Quantification of the percentage of γ-H2AX-positive HK-2 cells. Quantitative analysis of 5 random fields in tissue sections of 5 different mice. e Scratch assay of HK-2 cells treated with EVs. Scale bar represents 100 μm. f Quantification of the migration ratio of HK-2 cells in each group. g Immunostaining analysis for Ki67 in HK-2 cells treated with EVs for 24 h. Scale bar represents 100 μm. The data are presented as mean ± SEM. (n = 5; *P < 0.05 versus PBS, **P < 0.01 versus PBS)