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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Spatio-temporal model of Meox1 expression control involvement of Sca-1-positive stem cells in neointima formation through the synergistic effect of Rho/CDC42 and SDF-1α/CXCR4

Fig. 5

Meox1 triggered Sca-1-positive stem cell migration into neointimal through CXCR4. The experiment was designed to determine if the increased SDF-1α in injured vessels induced Sca-1-positive progenitor cell migration into neointima, using SDF-1α receptor CXCR4 blocker AMD3100 to affirm the role of SDF-1α on Sca-1-positive progenitor cell migration 7 days after vessel injury with or without AMD3100 application in vivo. A Immunofluorescence staining for CXCR4 in injury arteries for 7 days. Green fluorescence indicates CXCR4; blue fluorescence indicates DAPI-labeled nucleus. B–D Typical image and semi-quantitative analysis of double positive with CXCR4 were determined within media or intima of injured arteries treated with Ad-shMeox1 for 14 days by Image-Pro Plus software. Green fluorescence indicates CXCR4; blue fluorescence indicates DAPI-labeled nucleus. n = 6, *P < 0.05 vs. sham; #P < 0.05 vs. the injured arteries treated with Ad-Null. E, F Immunofluorescence staining of Sca-1 progenitor cells in neointima of injured arteries treated with AMD3100. Red fluorescence indicates Sca-1; blue fluorescence indicates DAPI-labeled nucleus. G–I AMD3100 decreased number of Sca-1-positive progenitor cells in neointima of E and F. n = 6, &P < 0.05 vs. the injured arteries treated with vehicles. I, neointima; M, media; A, adventitia

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Stem Cell Research & Therapy

ISSN: 1757-6512