Fig. 2

AM-MSCs and UCM-MSCs have different transcriptome profiles. a Hierarchical cluster analysis, and heatmap of differentially expressed genes (DEGs) between AM-MSCs (n = 3) and UCM-MSCs (n = 3). b Clustering of the samples based on distance according to RNA-seq analysis. The samples clustered origin-related. c The number of differentially expressed genes between AM-MSCs and UCM-MSCs represented by a diagram. A total of 2100 genes is more highly expressed in AM-MSCs and 1857 genes relatively low expressed. d GO-term enrichment analysis, based on biological process (BP), of genes, differentially expressed between AM-MSCs and UCM-MSCs. Significantly highly expressed genes in AM-MSCs are highlighted in red while blue represents DEGs in UCM-MSCs. e RT-qPCR analysis of pluripotency-related genes, OCT4, NANOG, and SOX2. f RT-qPCR analysis of inflammatory-related genes, IL-1β, TNF-α, IL-6, TGFβ1, and IL-10. The highly expressed genes TNF-α, and IL-6 are also categorized as stemness-related genes. GAPDH was used as an internal control. P-values < 0.05 were considered significant. *, P < 0.05; **, P < 0.01; ***, P < 0.001