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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Efficient hepatic differentiation and regeneration potential under xeno-free conditions using mass-producible amnion-derived mesenchymal stem cells

Fig. 4

Generation of hypo-immune potential universal cells from AM-MSCs. a The target DNA sequence in the human B2M locus is shown in red. b Schema of the process for generating universal donor AM-MSCs. After inducing B2M knockout, MHC I-negative AM-MSCs are selected by flow cytometry. c Sequencing of B2M in MHC I-KO and AM-MSCs after B2M knockout in AM-MSCs. d Expression of MSC CD markers (CD90, CD105) and hematopoietic markers (CD34) in B2M KO-AM-MSCs. e CD47 expression levels in AM-MSCs and B2M-KO AM-MSCs analyzed by flow cytometry. f Growth rate of AM-MSCs and B2M-KO-AM-MSCs. B2M-KO: B2M-KO-AM-MSCs. g The evaluation of PBMC proliferation assays (4 donors of PBMCs). All control groups were only cultured in a medium without MSC. Negative control: PBMC cultured without PHA, Positive control: PBMC cultured with PHA. NS: no significant, P-values < 0.05 were considered significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001

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Stem Cell Research & Therapy

ISSN: 1757-6512