Table 2 Immunohistochemistry and immunofluorescence antigen retrieval, primary and secondary antibodies
Antigen retrieval | Primary antibody | Secondary antibody |
|---|---|---|
10 mM sodium citrate (pH 6) | Wide spectrum screening cytokeratin (Pan-CK) (Z0622, Dako, Mulgrave, Victoria, Australia, 1:200) | Biotinylated goat anti-rabbit IgG (BA-100, Vector Laboratories, Meadowbrook, QLD, Australia, 1:150) |
Rabbit myeloperoxidase (MPO) (ab45977, Abcam, Cambridge, MA, USA, 1:100) | Donkey anti-rabbit 568 (Alexa Fluor conjugates, Life Technologies, Frederick, MD, USA, 1:500) | |
Rabbit CD45 (ab10558, Abcam, Cambridge, MA, USA, 1:100) | Goat anti-rabbit 647 (Alexa Fluor conjugates, Life Technologies, Frederick, MD, USA, 1:500) | |
Tris-EDTA buffer (pH 9) | Rat anti-neutrophil (NIMP-R14) (ab2557, Abcam, Cambridge, MA, USA, 1:100) | Goat anti-rat 488 (Alexa Fluor conjugates, Life Technologies, Frederick, MD, USA, 1:100) |
Rabbit recombinant anti-heme oxygenase 1 (HO-1) (ab52947, Abcam, Cambridge, MA, USA, 1:100) | Donkey anti-rabbit 568 (Alexa Fluor conjugates, Life Technologies, Frederick, MD, USA, 1:500) |