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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Hypoxic preconditioning rejuvenates mesenchymal stem cells and enhances neuroprotection following intracerebral hemorrhage via the miR-326-mediated autophagy

Fig. 6

Hypoxic preconditioning attenuates cellular senescence of OM-MSCs by upregulating miR-326 in vitro model. A Expression of miR-326 in OM-MSCs after transfection of mimics and inhibitors. B Under the hemin treatment, western blotting and quantitative analysis of the expression levels of p21, P53, and p16INK4A in normoxia OM-MSCs transfected with miRNA mimics control or miR-326 mimics. C Representative images of SA-β-gal staining and quantitative analysis of SA-β-gal-positive normoxia OM-MSCs transfected with miRNA mimics control or miR-326 mimics. D Representative images of SA-β-gal staining and quantitative analysis of SA-β-gal-positive hypoxia OM-MSCs transfected with miRNA inhibitor control or miR-326 inhibitor. E Western blotting and quantitative analysis of the expression levels of p21, P53, and p16INK4A in hypoxia OM-MSCs transfected with miRNA inhibitor control or miR-326 inhibitor. Data are expressed as the mean ± SEM (n = 3) (*P <0.05, **P <0.01, ***P <0.001)

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Stem Cell Research & Therapy

ISSN: 1757-6512