Skip to main content
Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Extracellular vesicles derived from mesenchymal stromal cells mediate endogenous cell growth and migration via the CXCL5 and CXCL6/CXCR2 axes and repair menisci

Fig. 6

Mechanistic analysis of chondrocyte cell growth and migration induced by MSC-EVs. A RT-qPCR analysis of CXCL5 and CXCL6 expression in chondrocytes treated with MSC-EVs (n = 6). The data shown represent the fold change relative to the control group. AKT and ERK were inhibited using 7 μM LY290042 and 20 μM PD98059, respectively. B Western blotting following AKT and ERK inhibition in response to MSC-EV treatment. C Chondrocytes were treated with MSC-EVs and an ERK inhibitor or a CXCR2 antagonist and then stained with crystal violet. D CCK-8 cell growth assay of chondrocytes treated with MSC-EVs and an ERK inhibitor or a CXCR2 antagonist. Absorbance corresponds to cell number. Measurements were repeated five times, and the average value was calculated (n = 6). E Transwell migration assay of chondrocytes treated with MSC-EVs and an ERK inhibitor or a CXCR2 antagonist. F Chondrocytes migrating to the lower surface were counted in five randomly selected fields (n = 6). Data are the mean ± SD. *P < 0.05 according to the Mann-Whitney U test

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512