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Table 2 Antibodies used in the study

From: EGFL6 regulates angiogenesis and osteogenesis in distraction osteogenesis via Wnt/β-catenin signaling

Antibody Purpose Dilution Product ID and manufacturer
Anti-Hif1a Western blot 1:2000 #14179, Cell Signaling Technology [CST], Danvers, MA, USA
Anti-VEGF-A Western blot 1:1000 ab1316, Abcam, Cambridge, UK
Anti-EMCN Western blot 1:1000 ab96315, Abcam, Cambridge, UK
Anti-CD31 Western blot 1:2000 ab32457, Abcam, Cambridge, UK
Anti-BMP2 Western blot 1:1000 ab214821, Abcam, Cambridge, UK
Anti-RUNX2 Western blot 1:2000 #8486, CST, Danvers, MA, USA
Anti-CXCR4 Western blot 1:2000 ab124824, Abcam, Cambridge, UK
Anti-p-β-catenin Western blot 1:2000 #9561, CST, Danvers, MA, USA
Anti-β-catenin Western blot 1:2000 #8480, CST, Danvers, MA, USA
Anti-active β-catenin Western blot 1:2000 #8814, CST, Danvers, MA, USA
Anti-p-GSK3β Western blot 1:2000 #5558, CST, Danvers, MA, USA
Anti-p-AKT Western blot 1:2000 #9271, CST, Danvers, MA, USA
Anti-AKT Western blot 1:2000 #9272, CST, Danvers, MA, USA
Anti-Erk1/2 Western blot 1:2000 AF0155, Affinity Biosciences Ltd., Cincinnati, OH, USA
Anti-GAPDH Western blot 1:2500 #5174, CST, Danvers, MA, USA
Anti-OCN IHC 1:200 ab198228, Abcam, Cambridge, UK
Anti-VEGF-A IHC 1:200 Ab1316, Abcam, Cambridge, UK
Anti-active β-catenin IHC 1:200 #8814, CST, Danvers, MA, USA
Anti-RUNX2 IHC 1:250 #8486, CST, Danvers, MA, USA
Anti-EMCN IHC 1:100 sc-65495, Santa Cruz Biotechnology Inc., Dallas, TX, USA
Anti-CD31 IHC 1:100 ab24590, Abcam, Cambridge, UK
Alexa Fluor® IHC 1:500 Jackson Research Inc., West Grove, PA, USA
  1. IHC immunohistochemistry