Table 2 Antibodies used in the study
Antibody | Purpose | Dilution | Product ID and manufacturer |
|---|---|---|---|
Anti-Hif1a | Western blot | 1:2000 | #14179, Cell Signaling Technology [CST], Danvers, MA, USA |
Anti-VEGF-A | Western blot | 1:1000 | ab1316, Abcam, Cambridge, UK |
Anti-EMCN | Western blot | 1:1000 | ab96315, Abcam, Cambridge, UK |
Anti-CD31 | Western blot | 1:2000 | ab32457, Abcam, Cambridge, UK |
Anti-BMP2 | Western blot | 1:1000 | ab214821, Abcam, Cambridge, UK |
Anti-RUNX2 | Western blot | 1:2000 | #8486, CST, Danvers, MA, USA |
Anti-CXCR4 | Western blot | 1:2000 | ab124824, Abcam, Cambridge, UK |
Anti-p-β-catenin | Western blot | 1:2000 | #9561, CST, Danvers, MA, USA |
Anti-β-catenin | Western blot | 1:2000 | #8480, CST, Danvers, MA, USA |
Anti-active β-catenin | Western blot | 1:2000 | #8814, CST, Danvers, MA, USA |
Anti-p-GSK3β | Western blot | 1:2000 | #5558, CST, Danvers, MA, USA |
Anti-p-AKT | Western blot | 1:2000 | #9271, CST, Danvers, MA, USA |
Anti-AKT | Western blot | 1:2000 | #9272, CST, Danvers, MA, USA |
Anti-Erk1/2 | Western blot | 1:2000 | AF0155, Affinity Biosciences Ltd., Cincinnati, OH, USA |
Anti-GAPDH | Western blot | 1:2500 | #5174, CST, Danvers, MA, USA |
Anti-OCN | IHC | 1:200 | ab198228, Abcam, Cambridge, UK |
Anti-VEGF-A | IHC | 1:200 | Ab1316, Abcam, Cambridge, UK |
Anti-active β-catenin | IHC | 1:200 | #8814, CST, Danvers, MA, USA |
Anti-RUNX2 | IHC | 1:250 | #8486, CST, Danvers, MA, USA |
Anti-EMCN | IHC | 1:100 | sc-65495, Santa Cruz Biotechnology Inc., Dallas, TX, USA |
Anti-CD31 | IHC | 1:100 | ab24590, Abcam, Cambridge, UK |
Alexa Fluor® | IHC | 1:500 | Jackson Research Inc., West Grove, PA, USA |