Anti-Hif1a
|
Western blot
|
1:2000
|
#14179, Cell Signaling Technology [CST], Danvers, MA, USA
|
Anti-VEGF-A
|
Western blot
|
1:1000
|
ab1316, Abcam, Cambridge, UK
|
Anti-EMCN
|
Western blot
|
1:1000
|
ab96315, Abcam, Cambridge, UK
|
Anti-CD31
|
Western blot
|
1:2000
|
ab32457, Abcam, Cambridge, UK
|
Anti-BMP2
|
Western blot
|
1:1000
|
ab214821, Abcam, Cambridge, UK
|
Anti-RUNX2
|
Western blot
|
1:2000
|
#8486, CST, Danvers, MA, USA
|
Anti-CXCR4
|
Western blot
|
1:2000
|
ab124824, Abcam, Cambridge, UK
|
Anti-p-β-catenin
|
Western blot
|
1:2000
|
#9561, CST, Danvers, MA, USA
|
Anti-β-catenin
|
Western blot
|
1:2000
|
#8480, CST, Danvers, MA, USA
|
Anti-active β-catenin
|
Western blot
|
1:2000
|
#8814, CST, Danvers, MA, USA
|
Anti-p-GSK3β
|
Western blot
|
1:2000
|
#5558, CST, Danvers, MA, USA
|
Anti-p-AKT
|
Western blot
|
1:2000
|
#9271, CST, Danvers, MA, USA
|
Anti-AKT
|
Western blot
|
1:2000
|
#9272, CST, Danvers, MA, USA
|
Anti-Erk1/2
|
Western blot
|
1:2000
|
AF0155, Affinity Biosciences Ltd., Cincinnati, OH, USA
|
Anti-GAPDH
|
Western blot
|
1:2500
|
#5174, CST, Danvers, MA, USA
|
Anti-OCN
|
IHC
|
1:200
|
ab198228, Abcam, Cambridge, UK
|
Anti-VEGF-A
|
IHC
|
1:200
|
Ab1316, Abcam, Cambridge, UK
|
Anti-active β-catenin
|
IHC
|
1:200
|
#8814, CST, Danvers, MA, USA
|
Anti-RUNX2
|
IHC
|
1:250
|
#8486, CST, Danvers, MA, USA
|
Anti-EMCN
|
IHC
|
1:100
|
sc-65495, Santa Cruz Biotechnology Inc., Dallas, TX, USA
|
Anti-CD31
|
IHC
|
1:100
|
ab24590, Abcam, Cambridge, UK
|
Alexa Fluor®
|
IHC
|
1:500
|
Jackson Research Inc., West Grove, PA, USA
|