Fig. 5

hucMSC-derived exosomes decrease the activation of NLRP3 inflammasomes in macrophages. a The quantitative analysis of IL-1β (pg ml⁻¹) in THP-1 cell supernatant by ELISA. b QRT-PCR analysis of the mRNA expression level of NLRP3 inflammasome-related molecules in THP-1 cells. c Western blot analysis of the protein expression level of NLRP3 inflammasome-related molecules in THP-1 cell lysate and grayscale scanning analysis. d IF analysis of ASC oligomerization in THP-1 cells (scale bar = 50 μm) and the statistical analysis of the percentage of ASC foci cells. e The quantitative analysis of IL-1β (pg ml⁻¹) in MPM supernatant by ELISA. f QRT-PCR analysis of the mRNA expression level of NLRP3 inflammasome-related molecules in MPMs. g Western blot analysis of the protein expression level of NLRP3 inflammasome-related molecules in MPMs lysate and grayscale scanning analysis. #P < 0.05, ##P < 0.01, ###P < 0.001 vs Ctrl by ANOVA; *P < 0.05, **P < 0.01, ***P < 0.001 vs LPS + NIG by ANOVA