Fig. 7
From: Low level laser therapy promotes bone regeneration by coupling angiogenesis and osteogenesis
LLLT activating ROS/HIF-α signaling pathway in co-culture system. A In HUVECs, hBMSCs, and co-cultured HUVECs and hBMSCs, H2O2 (50 μM, 30min) or vitamin C (100 μM, 24h) was added, respectively. The expression of HIF-1α was detected by Western blot. B Western blot and quantification of HIF-1α. C The expression of ROS (green) was detected by ROS assay after 7 days of LLLT (4.5 J/cm2, every day) in HUVECs, hBMSCs, and co-cultured HUVECs and hBMSCs, scale bar, 100 μm. D The quantification of ROS by mean optical density after 7 days of LLLT. E The expression of ROS (green) was detected after 21 days of LLLT. F The quantification of ROS after 21 days of LLLT, scale bar, 100 μm (ns, not statistically significant , *p < 0.05, **P < 0.01, ***P < 0.001)